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3d in vitro collagen model of ecm  (Cell Biolabs Inc)

 
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    Structured Review

    Cell Biolabs Inc 3d in vitro collagen model of ecm
    ( A ) Beta-aminopropionitrile (BAPN) <t>reduced</t> <t>collagen</t> crosslinking and insoluble collagen deposition into <t>ECM</t> after 3 weeks in culture. Both the amount of collagen deposited into the ECM and the concentration of the pyridinoline and deoxypyridinoline crosslinks were normalized to the total protein content in the sample. Data presented as mean ± SD. Kruskal–Wallis ANOVA, Dunn’s test; statistical significance: * p = 0.0252, ** p = 0.0028. ( B ) Second harmonic generation (SHG) imaging of collagen type I fibers after 3 weeks in culture. The decrease in the SHG signal depicted in green pseudocolor (upper panel) demonstrates the effect of BAPN on the correct structural assembly of collagen type I. The autofluorescence of the fibroblasts is depicted in red pseudocolor (lower panel). Leica DMi8 confocal microscope, obj. 63× water immersion, Scale bar = 50µm.
    3d In Vitro Collagen Model Of Ecm, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3d+in+vitro+collagen+model+of+ecm/3d+in+vitro+collagen+model+of+ecm/pmc08584281-221-8-18
    Average 90 stars, based on 1 article reviews
    3d in vitro collagen model of ecm - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Increased Collagen Crosslinking in Stiff Clubfoot Tissue: Implications for the Improvement of Therapeutic Strategies"

    Article Title: Increased Collagen Crosslinking in Stiff Clubfoot Tissue: Implications for the Improvement of Therapeutic Strategies

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms222111903

    ( A ) Beta-aminopropionitrile (BAPN) reduced collagen crosslinking and insoluble collagen deposition into ECM after 3 weeks in culture. Both the amount of collagen deposited into the ECM and the concentration of the pyridinoline and deoxypyridinoline crosslinks were normalized to the total protein content in the sample. Data presented as mean ± SD. Kruskal–Wallis ANOVA, Dunn’s test; statistical significance: * p = 0.0252, ** p = 0.0028. ( B ) Second harmonic generation (SHG) imaging of collagen type I fibers after 3 weeks in culture. The decrease in the SHG signal depicted in green pseudocolor (upper panel) demonstrates the effect of BAPN on the correct structural assembly of collagen type I. The autofluorescence of the fibroblasts is depicted in red pseudocolor (lower panel). Leica DMi8 confocal microscope, obj. 63× water immersion, Scale bar = 50µm.
    Figure Legend Snippet: ( A ) Beta-aminopropionitrile (BAPN) reduced collagen crosslinking and insoluble collagen deposition into ECM after 3 weeks in culture. Both the amount of collagen deposited into the ECM and the concentration of the pyridinoline and deoxypyridinoline crosslinks were normalized to the total protein content in the sample. Data presented as mean ± SD. Kruskal–Wallis ANOVA, Dunn’s test; statistical significance: * p = 0.0252, ** p = 0.0028. ( B ) Second harmonic generation (SHG) imaging of collagen type I fibers after 3 weeks in culture. The decrease in the SHG signal depicted in green pseudocolor (upper panel) demonstrates the effect of BAPN on the correct structural assembly of collagen type I. The autofluorescence of the fibroblasts is depicted in red pseudocolor (lower panel). Leica DMi8 confocal microscope, obj. 63× water immersion, Scale bar = 50µm.

    Techniques Used: Concentration Assay, Imaging, Microscopy

    Related Articles

    In Vitro:

    Article Title: Increased Collagen Crosslinking in Stiff Clubfoot Tissue: Implications for the Improvement of Therapeutic Strategies
    Article Snippet: Leica non-descanned HyD detectors and a 430/24 band-pass filter were used for collecting SHG signals, and Leica non-descanned HyD detectors and a 610/75 band-pass filter were used for collecting autofluorescence signals. .. A three-dimensional (3D) in vitro collagen model of ECM (CytoSelect 24-Well Cell Contraction Assay Kit—floating matrix model, CBA-5020, Cell Biolabs, San Diego, CA, USA) was used to describe the potential of BAPN to reduce ECM contractility. ..

    Contraction Assay:

    Article Title: Increased Collagen Crosslinking in Stiff Clubfoot Tissue: Implications for the Improvement of Therapeutic Strategies
    Article Snippet: Leica non-descanned HyD detectors and a 430/24 band-pass filter were used for collecting SHG signals, and Leica non-descanned HyD detectors and a 610/75 band-pass filter were used for collecting autofluorescence signals. .. A three-dimensional (3D) in vitro collagen model of ECM (CytoSelect 24-Well Cell Contraction Assay Kit—floating matrix model, CBA-5020, Cell Biolabs, San Diego, CA, USA) was used to describe the potential of BAPN to reduce ECM contractility. ..

    Crocin Bleaching Assay:

    Article Title: Increased Collagen Crosslinking in Stiff Clubfoot Tissue: Implications for the Improvement of Therapeutic Strategies
    Article Snippet: Leica non-descanned HyD detectors and a 430/24 band-pass filter were used for collecting SHG signals, and Leica non-descanned HyD detectors and a 610/75 band-pass filter were used for collecting autofluorescence signals. .. A three-dimensional (3D) in vitro collagen model of ECM (CytoSelect 24-Well Cell Contraction Assay Kit—floating matrix model, CBA-5020, Cell Biolabs, San Diego, CA, USA) was used to describe the potential of BAPN to reduce ECM contractility. ..



    Similar Products

    3d in vitro collagen model of ecm  (Cell Biolabs Inc)
    90
    Cell Biolabs Inc 3d in vitro collagen model of ecm
    ( A ) Beta-aminopropionitrile (BAPN) <t>reduced</t> <t>collagen</t> crosslinking and insoluble collagen deposition into <t>ECM</t> after 3 weeks in culture. Both the amount of collagen deposited into the ECM and the concentration of the pyridinoline and deoxypyridinoline crosslinks were normalized to the total protein content in the sample. Data presented as mean ± SD. Kruskal–Wallis ANOVA, Dunn’s test; statistical significance: * p = 0.0252, ** p = 0.0028. ( B ) Second harmonic generation (SHG) imaging of collagen type I fibers after 3 weeks in culture. The decrease in the SHG signal depicted in green pseudocolor (upper panel) demonstrates the effect of BAPN on the correct structural assembly of collagen type I. The autofluorescence of the fibroblasts is depicted in red pseudocolor (lower panel). Leica DMi8 confocal microscope, obj. 63× water immersion, Scale bar = 50µm.
    3d In Vitro Collagen Model Of Ecm, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3d+in+vitro+collagen+model+of+ecm/3d+in+vitro+collagen+model+of+ecm/pmc08584281-221-8-18
    Average 90 stars, based on 1 article reviews
    3d in vitro collagen model of ecm - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Beta-aminopropionitrile (BAPN) reduced collagen crosslinking and insoluble collagen deposition into ECM after 3 weeks in culture. Both the amount of collagen deposited into the ECM and the concentration of the pyridinoline and deoxypyridinoline crosslinks were normalized to the total protein content in the sample. Data presented as mean ± SD. Kruskal–Wallis ANOVA, Dunn’s test; statistical significance: * p = 0.0252, ** p = 0.0028. ( B ) Second harmonic generation (SHG) imaging of collagen type I fibers after 3 weeks in culture. The decrease in the SHG signal depicted in green pseudocolor (upper panel) demonstrates the effect of BAPN on the correct structural assembly of collagen type I. The autofluorescence of the fibroblasts is depicted in red pseudocolor (lower panel). Leica DMi8 confocal microscope, obj. 63× water immersion, Scale bar = 50µm.

    Journal: International Journal of Molecular Sciences

    Article Title: Increased Collagen Crosslinking in Stiff Clubfoot Tissue: Implications for the Improvement of Therapeutic Strategies

    doi: 10.3390/ijms222111903

    Figure Lengend Snippet: ( A ) Beta-aminopropionitrile (BAPN) reduced collagen crosslinking and insoluble collagen deposition into ECM after 3 weeks in culture. Both the amount of collagen deposited into the ECM and the concentration of the pyridinoline and deoxypyridinoline crosslinks were normalized to the total protein content in the sample. Data presented as mean ± SD. Kruskal–Wallis ANOVA, Dunn’s test; statistical significance: * p = 0.0252, ** p = 0.0028. ( B ) Second harmonic generation (SHG) imaging of collagen type I fibers after 3 weeks in culture. The decrease in the SHG signal depicted in green pseudocolor (upper panel) demonstrates the effect of BAPN on the correct structural assembly of collagen type I. The autofluorescence of the fibroblasts is depicted in red pseudocolor (lower panel). Leica DMi8 confocal microscope, obj. 63× water immersion, Scale bar = 50µm.

    Article Snippet: A three-dimensional (3D) in vitro collagen model of ECM (CytoSelect 24-Well Cell Contraction Assay Kit—floating matrix model, CBA-5020, Cell Biolabs, San Diego, CA, USA) was used to describe the potential of BAPN to reduce ECM contractility.

    Techniques: Concentration Assay, Imaging, Microscopy